What precautions should be taken while using a pipette?

What precautions should be taken while using a pipette?

Take Care of Your Pipette

  • Have Your Pipette Serviced Every 6–12 Months.
  • Check Your Pipette for Damage Daily.
  • Clean Your Pipette Each Day Before Use.
  • Store Your Pipette Vertically, Using a Pipette Holder.
  • Never Put Your Pipette on Its Side With Liquid in the Tip.
  • Use Well-Fitting Tips.

What should be avoided when using a micropipette?

-Never rotate the volume adjustor beyond the upper or lower range of the pipette, as stated by the manufacturer. -Never invert or lay the micropipettor down with a filled tip; fluid could run back into the piston. -Never let the plunger snap back after withdrawing or expelling fluid; this could damage the piston.

What should you always remember to do when using a micropipette?

Steps to follow when using a micropipette

  1. Select the volume.
  2. Set the tip.
  3. Press and hold the plunger at the first stop.
  4. Place the tip in the liquid.
  5. Slowly release the plunger.
  6. Pause for a second and then move the tip.
  7. Insert the tip into the delivery vessel.
  8. Press the plunger to the second stop.

What are the dos and don’ts when using a micropipette?

-Do not attached tip by hands, even with gloves. 2- Do not turn the pippete on its side when there is liquid in the tip. 3-Do not clean tips for REUSE, as their metrological characteristics will no longer be reliable. 4- Don’t contaminate you or the pipette.

What are three important precautions in micropipette use?

Take the following precautions when using a micropipette: run back into the piston. ➢ NEVER let the plunger snap back after withdrawing or expelling fluid; this could damage the piston. ➢ NEVER immerse the barrel of the micropipettor in fluid. ➢ NEVER flame the tip of the micropipettor.

How do you handle a pipette?

Holding the pipette at an angle as it is removed from the liquid alters the aspirated volume. Hold the pipette loosely and utilize the finger rest. Remember to return the pipette to the pipette stand between deliveries. Avoid handling pipette tips or reservoirs with bare hands.

What are the best practices when using a micropipette?

Best Practices for the Use of Micropipettes

  • Evaporation, heat transfer and changes in barometric pressure.
  • First stop to aspirate, second stop to dispense.
  • Go with the flow.
  • Precision and accuracy are directly related to technique.
  • Equipment choice makes a measurable difference.

Why do we use micropipettes describe appropriate use of a micropipette?

A micropipette is a common yet an essential laboratory instrument used to accurately and precisely transfer volumes of liquid in the microliter range. Micropipettes are available in single channel and multi channel variants.

What do you use a pipette for?

To transport a measured volume of liquid

What are the safety precautions when using a pipette?

Safety Precautions for Using a Pipette 1 Mouth Pipetting. The basic glass pipette requires applied suction to draw up a solution. 2 Broken Glass. To work a glass pipette, you use a suction bulb to produce draw inside the tube. 3 Overfilled Pipettes. 4 Repetitive Strain.

What should I never adjust the volume of my micropipette?

Never adjust the volume beyond the range of the micropipette. No micropipette should be adjusted below zero µL. The P20 should never be adjusted above 20 µL, the P200 over 200 µL and the P1000 over 1 mL. Never force the volume adjuster to dial.

Can the tip of a micropipette touch the receiving liquid?

Do not allow the tip of your micropipette to touch the receiving liquid. Some of the liquid may be drawn back up into the micropipette, contaminating your sample for the remainder of the micropipette sequence. Did you find this page helpful? What Is the Purpose of a Pipette?

What are the risks associated with using a specificpipette?

Pipettes are widely used for many applications and are therefore exposed to diverse types of specimens and reagents. They introduce quality-specific concerns regarding accuracy and precision, and can also introduce safety risks, especially when the technologist is working with hazardous chemicals or specimens with a high infection risk.