Why is NaCl used in DNA extraction?

Why is NaCl used in DNA extraction?

Sodium chloride helps to remove proteins that are bound to the DNA. It also helps to keep the proteins dissolved in the aqueous layer so they don’t precipitate in the alcohol along with the DNA. Ethanol or isopropyl alcohol causes the DNA to precipitate.

Why is sodium acetate used in DNA precipitation by ethanol rather than sodium chloride?

DNA in ethanol precipitates better in the presence of salt. NaAc (or KAc) is well soluble in ethanol, as opposed to for example NaCl, thats why it is used for precipitation. An ethanol precipitation with sodium acetate is a concentration/ wash step, it is not a purification step.

What is the purpose of adding detergent to extract DNA?

During a DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the DNA is released into solution. Then alcohol added to the solution causes the DNA to precipitate out.

Why is chilled ethanol used for DNA extraction?

Using ice-cold ethanol and ice-coldwater increases the yield of DNA. Low temperatures protect the DNA by slowing down the activity of enzymes that could break it apart. DNases in the cytoplasm would destroy the DNAof viruses entering the cell. Cold ethanol helps the DNA to precipitate more quickly.

Why is ethanol used to precipitate DNA?

DNA is polar due to its highly charged phosphate backbone. If enough ethanol is added, the electrical attraction between phosphate groups and any positive ions present in solution becomes strong enough to form stable ionic bonds and DNA precipitation.

Why chilled ethanol is used in DNA extraction?

Why tris buffer is used in protein purification?

Tris-HCl – With an effective pH range of 7.0 to 9.0, this buffer is capable of extracting soluble cytoplasmic proteins. The pH of tris buffers is highly dependent on the temperature and the concentration of the solution.

Why Phenol is used in DNA extraction?

Extraction of DNA containing samples with acidic phenol results in the denaturation of the DNA, and once denatured, the DNA partitions to the organic phase. This is a key feature of many RNA purification protocols, which is one of the reasons acidic buffer-saturated phenol is used.