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What is the proper technique for staining a wet mount?

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What is the proper technique for staining a wet mount?

A wet mount requires a liquid, tweezers, pipette and paper towels….A simple staining method:

  1. Add a drop of staining solution on the edge of one side of the cover slip.
  2. Position the edge of a paper towel on the opposite end.
  3. Allow dye to be pulled across the specimen.

What is the proper way of preparing a wet mount slide?

In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. Place a sample on the slide. Using a pipette, place a drop of water on the specimen. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent.

When making a wet mount how should you lower the coverslip?

Using the tweezers, place the specimen in the middle of the drop. While holding the cover slip upright, carefully place one edge of the cover slip next to the water. Slowly lower the upper edge of the cover slip onto the water. The objective is to minimize or eliminate air bubbles under the cover slip.

Why do you put a coverslip on your wet mount smear?

Without the cover in place, surface tension would cause the droplet to bunch up in a dome. The cover breaks this tension, flattening the sample and allowing very close inspection with minimal focusing. The cover also serves to protect the objective lens from interfering with the sample drop.

How do you put a coverslip on a slide?

Starts here7:54Coverslipping Slides by Hand Techniques – YouTubeYouTube

How do you prepare a wet mount?

How to make a wet-mount slide

  1. Collect a thin slice of your sample and place it on a clean, dry slide.
  2. Place one drop of water over your sample.
  3. Place the coverslip at a 45-degree angle with one edge touching the water and let go.
  4. Your slide is ready to be viewed.

What is hanging drop method?

Hanging drop method is the traditional method for examining the cell motility and morphology by taking the living microorganisms from the liquid media. It makes the use of glass slides with one small concave depression towards the centre, a coverslip, petroleum jelly, microbial suspension and sterile inoculating loop.