Table of Contents
- 1 Why Sodium citrate is not used in CBC?
- 2 Can CBC be done from citrate tube?
- 3 How does EDTA work as an anticoagulant?
- 4 How does citrate keep blood from clotting?
- 5 Why Heparin is not used for CBC?
- 6 Why EDTA is not used for coagulation studies?
- 7 What happens if a sample has clotted in the EDTA tube?
- 8 Do citrated blood samples differ from those mixed wtih dried EDTA?
Why Sodium citrate is not used in CBC?
It can be concluded that samples treated with Sodium Citrate results in significant change in blood parameters. Therefore, it is better to use Heparin and EDTA than Sodium Citrate for complete blood count (CBC).
Can CBC be done from citrate tube?
To report CBC data from a citrate tube only, apply the 1.1 correction factor to all parameters reported per unit volume, which includes the red blood cell count, WBC count, platelet count, hemoglobin, and absolute counts for all cell types in the WBC differential.
Why is EDTA tube used for CBC?
Anticoagulants are used to prevent clot formation both in vitro and in vivo. Historically, EDTA has been recommended as the anticoagulant of choice for hematological testing because it allows the best preservation of cellular components and morphology of blood cells.
What is the difference between sodium citrate and EDTA?
Sodium Citrate (Light Blue) Like EDTA, citrate acts by removing calcium from the blood. Unlike EDTA, it’s reversible—so calcium can be added back to study coagulation under controlled conditions. Citrated plasma is also used to measure coagulation-relevant factors.
How does EDTA work as an anticoagulant?
Ethylenediaminetetraacetic acid (EDTA) strongly and irreversibly chelates (binds) calcium ions, preventing blood from clotting. Citrate is in liquid form in the tube and is used for coagulation tests, as well as in blood transfusion bags. It binds the calcium, but not as strongly as EDTA.
How does citrate keep blood from clotting?
Citrate is usually administered as ACD-A, but other forms are available (i.e. ACD-B and trisodium citrate), and it prevents coagulation by binding ionized calcium, which is required in clot formation.
Why is EDTA not used as an anticoagulant?
Because EDTA’s anticoagulant effect is practically non-reversible, while that of sodium citrate is. Dear It is because EDTA has chelation effect on the calcium affecting clotting time More than citrate.
Why is heparin tube not suitable for CBC and coagulation?
Heparin (green top tube) is not recommended as an anticoagulant for cell counts, because the cells clump in heparin, invalidating counts. Citrate (blue top tube) is not recommended due to the dilution of the blood by the liquid citrate. Ensure that the blood is mixed promptly with the EDTA to avoid sample clotting.
Why Heparin is not used for CBC?
Heparin is not recommended as an anticoagulant because leukocytes and thrombocytes clump, invalidating WBC counts and differential cell counts.
Why EDTA is not used for coagulation studies?
EDTA binds calcium Ions more strongly than Citrate, EDTA also binds structural calcium in the Proteins, partly inactivating them. I have read on one of the wbsites that EDTA is not recommended for Coagulation studies because of its chelation properties as it keeps removing Ca ions even if more calcium is added.
Why EDTA is not used for coagulation?
EDTA is not recommended for coagulation studies .. because, there is rapid lost of 2 factors which is very important in coagulation mechanism (VIII and V) called labile factor another matter the calcium would precipitated.
How much does EDTA affect citrate count?
The decreases in citrate were significant (18–30%, P < 0.001). The addition of EDTA (1.5 mg/ml) to the citrated samples after the sixth hour count created a significant rise (6–22%, P < 0.01) in the counts between the sixth and the seventh hour.
What happens if a sample has clotted in the EDTA tube?
If blood has clotted in the EDTA tube, the sample will not be analyzed. Clotting affects our automated hematology analyzer adversely and also invalidates cell counts in an unpredictable fashion. For CUHA, we make every effort to notify the clinician/technician/student that a sample has clotted so that a new sample can be drawn from that patient.
Do citrated blood samples differ from those mixed wtih dried EDTA?
Our observations show that platelet counts in citrated blood samples are lower than those in EDTA and highlight the necessity to present citrated samples mixed wtih dried EDTA when characterization or quality control of blood and blood components is required.
Why is EDTA not recommended for coagulation studies?
I have read on one of the wbsites that EDTA is not recommended for Coagulation studies because of its chelation properties as it keeps removing Ca ions even if more calcium is added. Because EDTA’s anticoagulant effect is practically non-reversible, while that of sodium citrate is.