Table of Contents
- 1 What are the advantages of using the Kirby Bauer method?
- 2 When do you use the Kirby Bauer test?
- 3 What does the Kirby-Bauer test assess quizlet?
- 4 What would cause growth of colonies within a zone of inhibition?
- 5 What is special about Mueller Hinton agar that makes it useful for the Kirby-Bauer Test?
- 6 What does the Kirby-Bauer Test assess quizlet?
What are the advantages of using the Kirby Bauer method?
The microbroth dilution method has become more widely used due to its ability to become automated, however, the Kirby Bauer method may be able to detect subpopulations of resistant bacteria that would be visualized as inner colonies in the zone of diffusion.
When do you use the Kirby Bauer test?
The Kirby-Bauer test, known as the disk-diffusion method, is the most widely used antibiotic susceptibility test in determining what choice of antibiotics should be used when treating an infection. This method relies on the inhibition of bacterial growth measured under standard conditions.
What is the Kirby Bauer test used for?
The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds in order to assist a physician in selecting treatment options for his or her patients.
What are the standard procedures required for the Kirby Bauer method?
In Kirby-Bauer testing, bacteria are placed on a plate of solid growth medium and wafers of antibiotics (white disks, shown) are added to the plate. After allowing the bacteria to grow overnight, areas of clear media surrounding the disks indicate that the antibiotic inhibits bacterial growth.
What does the Kirby-Bauer test assess quizlet?
What is the Kirby-Bauer Test used for? Uses antibiotic-containing discs to measure the sensitivity of bacteria to antibiotics by culturing bacteria on a plate and placing the soaked discs on the bacteria. Zone of inhibition is then measured to calculate the sensitivity or resistance of the bacteria.
What would cause growth of colonies within a zone of inhibition?
Colonies that grow within a zone of inhibition are NOT susceptible to the antibiotic used at that particular concentration. This is one way in which you can select for resistant mutants to the specific antibiotic. It is also possible that your plate is contaminated by a non-susceptible strain.
Why is the Zone of Inhibition important?
Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms. In the world of antimicrobial substances/surfaces, the degree to which these materials are inhibitory can be of vital importance to the health of the consumer.
What is the preferred medium for use with the Kirby-Bauer susceptibility test?
The Kirby-Bauer disk diffusion method is one of the most widely practiced antimicrobial susceptibility tests (AST). It is affected by many factors among which are the media used. Mueller-Hinton agar (MHA) is the standard medium recommended in guidelines.
What is special about Mueller Hinton agar that makes it useful for the Kirby-Bauer Test?
It is a non-selective, non-differential medium. This means that almost all organisms plated on here will grow. It contains starch. Starch is known to absorb toxins released from bacteria, so that they cannot interfere with the antibiotics.
What does the Kirby-Bauer Test assess quizlet?
What are the factors affecting the results of the Kirby-Bauer method?
The disc diffusion techniques most commonly used (the Kirby-Bauer and Stokes’s tests) take account of important factors, including the inoculum density, the composition of the medium, the delay between application of the disc and incubation, the temperature ofincubation, etc., but the resulting zone of inhibition is …
How can the Kirby-Bauer Test be used to determine if an antibiotic is broad spectrum?
Figure: Kirby-Bauer test: In Kirby–Bauer testing, discs containing antibiotics are placed on agar where bacteria are growing, and the antibiotics diffuse out into the agar. If an antibiotic stops the bacteria from growing, one can see circular areas around the wafers where bacteria have not grown.